Lck Antibody (YA4947)

Lck Antibody (YA4947) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Lck. It can be applicated for WB, ELISA assays, in the background of human.

Lck Protein, a non-receptor tyrosine-protein kinase, plays a crucial role in the selection and maturation of developing T-cells in the thymus and the function of mature T-cells. It is a key player in T-cell antigen receptor (TCR)-linked signal transduction pathways, constitutively associated with the cytoplasmic portions of CD4 and CD8 surface receptors. Upon TCR interaction with a peptide antigen-bound MHC complex, CD4 and CD8 associate with MHC class II and class I molecules, respectively, recruiting Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Upon stimulation, the TCR recruits the tyrosine kinase ZAP70, leading to lymphokine production. Lck also contributes to signaling by other receptors, directly associating with the cytoplasmic tail of CD2, resulting in hyperphosphorylation and activation of Lck. Furthermore, Lck plays a role in the IL2 receptor-linked signaling pathway, and its increased activity follows IL2 binding to its receptor, influencing the T-cell proliferative response. Expressed at all stages of thymocyte development, Lck is essential for regulating maturation events governed by both pre-TCR and mature alpha beta TCR, and it phosphorylates various substrates, including RUNX3, PTK2B/PYK2, MAPT, RHOH, TYROBP, and interacts with FYB2.

P06239

Predicted band size: 58 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human Lck expressed in E. Coli.