LATS1 Antibody (YA5996)

LATS1 Antibody (YA5996) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LATS1. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

LATS1 is a negative regulator of YAP1 in the Hippo signaling pathway, playing a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway consists of a kinase cascade where STK3/MST2 and STK4/MST1, in complex with SAV1, phosphorylate and activate LATS1/2 in complex with MOB1, which then phosphorylates and inactivates the oncoproteins YAP1 and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its nuclear translocation, thereby regulating genes important for cell proliferation, death, and migration. As a tumor suppressor, LATS1 maintains ploidy through its roles in mitotic progression and the G1 tetraploidy checkpoint. It negatively regulates G2/M transition by downregulating CDK1 kinase activity and is involved in controlling p53 expression. Additionally, LATS1 affects cytokinesis by negatively modulating LIMK1 to regulate actin polymerization. It may also function in endocrine processes and promotes mammary gland epithelial cell differentiation through both the Hippo pathway and intracellular estrogen receptor signaling by facilitating ESR1 degradation. Recent studies show LATS1 activates the NLRP3 inflammasome by mediating phosphorylation of NLRP3 at Ser-265 following its palmitoylation.

O95835

Predicted band size: 127 kDa; Observed band size: 140 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG