Keap1 Antibody (YA6097)

Keap1 Antibody (YA6097) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Keap1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

KEAP1, as the substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, intricately regulates the cellular response to oxidative stress by orchestrating the ubiquitination of NFE2L2/NRF2. Serving as a crucial sensor for oxidative and electrophilic stress, KEAP1, under normal conditions, facilitates the ubiquitination and subsequent degradation of NFE2L2/NRF2, a transcription factor essential for the expression of numerous cytoprotective genes. When confronted with oxidative stress, distinct electrophile metabolites induce non-enzymatic covalent modifications on highly reactive cysteine residues in KEAP1, effectively dampening the ubiquitin ligase activity of the BCR(KEAP1) complex. This disruption promotes the nuclear accumulation of NFE2L2/NRF2 and triggers the expression of phase II detoxifying enzymes. Furthermore, selective autophagy leads to the sequestration of KEAP1 in inclusion bodies through its interaction with SQSTM1/p62, resulting in the inactivation of the BCR(KEAP1) complex and the activation of NFE2L2/NRF2. Notably, the BCR(KEAP1) complex extends its ubiquitin ligase activity to substrates like SQSTM1/p62, BPTF, and PGAM5, modulating their degradation via the proteasome. The ubiquitin ligase activity of the BCR(KEAP1) complex faces inhibition in response to oxidative stress and electrophile metabolites such as sulforaphane, as these metabolites react with reactive cysteine residues in KEAP1, leading to the non-enzymatic covalent modifications that incapacitate the complex. Moreover, selective autophagy contributes to the inactivation of the BCR(KEAP1) complex through the interaction between KEAP1 and SQSTM1/p62, promoting the sequestration of the complex in inclusion bodies and facilitating its degradation.

Q14145

Predicted band size: 70 kDa; Observed band size: 60-70 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG