JNK2 Antibody (YA5409)

JNK2 Antibody (YA5409) is a mouse-derived and non-conjugated monoclonal antibody, targeting to JNK2. It can be applicated for IHC-P assays, in the background of human, mouse, rat.

JNK2, a serine/threonine-protein kinase, is a central player in diverse cellular processes, encompassing cell proliferation, differentiation, migration, transformation, and programmed cell death. Activation of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway by extracellular stimuli, such as pro-inflammatory cytokines or physical stress, involves the phosphorylation and activation of JNK2 by dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7. Within this cascade, JNK2 phosphorylates critical transcription factors, particularly components of AP-1 like JUN and ATF2, thereby modulating AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, JNK2 inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Additionally, JNK2 plays a crucial role in stressed cell apoptosis by phosphorylating key regulatory factors, including TP53 and YAP1. In T-cells, JNK2, along with MAPK8, is essential for the polarized differentiation of T-helper cells into Th1 cells. Furthermore, upon T-cell receptor (TCR) stimulation, JNK2 is activated to regulate JUN protein levels by interacting with CARMA1, BCL10, MAP2K7, and MAP3K7/TAK1. JNK2 is also instrumental in osmotic stress-induced disruption of epithelial tight junctions and, when activated, promotes beta-catenin/CTNNB1 degradation, thereby inhibiting the canonical Wnt signaling pathway. Additionally, JNK2 participates in neurite growth in spiral ganglion neurons and phosphorylates the CLOCK-BMAL1 heterodimer, contributing to the regulation of the circadian clock. Notably, JNK2 isoforms exhibit distinct binding patterns, with alpha-1 and alpha-2 preferentially binding to JUN, while beta-1 and beta-2 bind to ATF2. However, phosphorylation efficiency remains consistent across all isoforms, and JUNB is not a substrate for JNK2 alpha-2, with JUND binding only weakly to it.

P45984

Observed band size: 46,54 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

IHC: 1:50-200

IHC-P

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthesized peptide derived from human JNK2