IκB-α Antibody (YA5889)

IκB-α Antibody (YA5889) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IκB-α. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

IkB alpha, a pivotal regulator in cellular responses, exerts its inhibitory influence by sequestering dimeric NF-kappa-B/REL complexes in the cytoplasm, thereby impeding their nuclear translocation and subsequent activation of transcription. This intricate control involves masking the nuclear localization signals of RELA/p65 and NFKB1/p50. Upon cellular stimulation by immune and pro-inflammatory cues, IkB alpha undergoes phosphorylation, triggering ubiquitination and degradation. This orchestrated process liberates the dimeric RELA, allowing its translocation to the nucleus for transcriptional activation. IkB alpha engages in a complex interplay with various proteins, such as RELA, NKIRAS1, NKIRAS2, RWDD3 isoforms, PRMT2, PRKACA, MEFV, and DDRGK1, highlighting its versatile interactions and regulatory roles in diverse cellular pathways.

P25963

Predicted band size: 36 kDa; Observed band size: 36 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

Human, Mouse, Rat

IHC: 1:200-1:2000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, ELISA

WB, IHC-P

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG