ISG15 Antibody (YA6221)

ISG15 Antibody (YA6221) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ISG15. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human.

ISG15 is a ubiquitin-like protein that plays a pivotal role in innate immune responses to viral infection through ISGylation (conjugation to target proteins) or as a free protein. ISGylation involves an enzymatic cascade (E1, E2, E3) that attaches ISG15 to lysine residues of targets like IFIT1, MX1/MxA, and PPM1B. For instance, ISGylation of the viral sensor IFIH1/MDA5 triggers oligomerization and immunity against coronaviruses, flaviviruses, and picornaviruses. ISG15 also modifies EIF2AK2/PKR (activating it), RIGI (inhibiting antiviral signaling), and EIF4E2 (enhancing translation suppression), while exhibiting broad antiviral activity against influenza A, HIV-1, and Ebola virus. The secreted form induces NK cell proliferation, acts as a neutrophil chemoattractant, and via ITGAL/ITGB2 receptor signaling, activates SRC kinases (LYN, HCK, FGR) to stimulate IFNG/IL10 secretion. by similar: ISG15 disrupts HIV-1 budding by blocking Gag-TSG101 interaction and inhibits Ebola VP40 ubiquitination by NEDD4 to suppress viral release.

P05161

Predicted band size: 18 kDa; Observed band size: 18 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human

WB: 1:500-1:2000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200,

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG