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  4. Integrin Linked ILK Antibody (YA3111)

Integrin Linked ILK Antibody (YA3111)

Cat. No.: HY-P83366
COA User Guide for Antibodies Technical Support

Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Integrin Linked ILK Antibody (YA3111) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin Linked ILK.

Background

The related gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of The protein is important in the epithelial to mesenchymal transition, and over-expression of The related gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.

Gene ID
SwissProt ID
Synonyms
ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK
Molecular Weight

Predicted band size: 51 kDa; Observed band size: 51 kDa

Purity

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Dilution Ratio

WB: 1/500-1/1000;IP: 1/20

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and U2OS (lane 2, 15 μg) using Integrin Linked ILK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IP

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

A synthetic peptide of human Integrin linked ILK

Database
Documentation

Integrin Linked ILK Antibody (YA3111) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Integrin Linked ILK Antibody (YA3111)
Cat. No.:
HY-P83366
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