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  5. IKK alpha + IKK beta Antibody (YA349)

IKK alpha + IKK beta Antibody (YA349)

Cat. No.: HY-P80414
COA
SDS
User Guide for Antibodies Technical Support

IKK alpha + IKK beta Antibody (YA349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK alpha + IKK beta.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IKK alpha + IKK beta Antibody (YA349) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK alpha + IKK beta.
Background

IKKβ protein, a serine kinase, plays a pivotal role in the NF-kappa-B signaling pathway, activated by diverse stimuli such as inflammatory cytokines, bacterial or viral products, DNA damage, or cellular stresses. As a constituent of the canonical IKK complex, IKKβ contributes to the conventional NF-kappa-B activation pathway. It phosphorylates inhibitors of NF-kappa-B on critical serine residues, facilitating their polyubiquitination and subsequent degradation by the proteasome. This orchestrated modification unleashes free NF-kappa-B, allowing its translocation into the nucleus and activation of the transcription of numerous genes involved in immune response, growth control, or protection against apoptosis. Beyond NF-kappa-B inhibitors, IKKβ phosphorylates various signaling pathway components, including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. These phosphorylations exert a negative regulation on canonical IKKs and may prevent the excessive production of inflammatory mediators. Moreover, IKKβ targets substrates such as FOXO3, NAA10, NCOA3, BCL10, IRS1, RIPK1, and IRF5, influencing diverse cellular processes and responses.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 87 kDa; Observed band size: 87 kDa
Purity

Protein A affinity purified.
Subcellular Location

Cytoplasm, Nucleus, Membrane raft.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1:500-1:1,000 ;ICC/IF: 1:100-1:500 ;IP: Use at an assay dependent concentration.

  • Western blot analysis of extracts from 293T (lane 1) and M-spleen (lane 2) and RAW264.7 (lane 3) using IKK alpha + IKK beta antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ICC/IF, IP
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human IKK beta.AA range:450-652.
Database

Entrez Gene: 1147 Human ; 3551 Human ; 12675 Mouse ; 16150 Mouse ;

SwissProt: O14920 Human ; O15111 Human ; O88351 Mouse ; Q60680 Mouse ;

OMIM: 615592 Human

Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

IKK alpha + IKK beta Antibody (YA349) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IKK alpha + IKK beta Antibody (YA349)
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