IGFBP6 Antibody (YA3076)

IGFBP6 Antibody (YA3076) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP6.

Enables identical protein binding activity and insulin-like growth factor II binding activity. Involved in cell migration and positive regulation of stress-activated MAPK cascade. Part of insulin-like growth factor binary complex. Implicated in obesity. Biomarker of breast cancer; in situ carcinoma; leiomyoma; and neovascular inflammatory vitreoretinopathy.

P24592

Predicted band size: 25 kDa; Observed band size: 35 kDa

Affinity Purified

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000

• 
  Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg), U-87MG (lane 2, 15 μg), Hela (lane 3, 15 μg), K562 (lane 4, 15 μg), and NCI-H1299 (lane 5, 15 μg) using IGFBP6 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human IGFBP6