HspA4 Antibody (YA3172)

HspA4 Antibody (YA3172) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3172), targeting HspA4, with a predicted molecular weight of 94 kDa (observed band size: 110 kDa). HspA4 Antibody (YA3172) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.

HSP70 is a crucial cytoprotective protein upregulated in response to temperature increases and various stresses. Its gene expression is mediated by the transcription factor HSF1, which binds to HSE elements in the hsp70 promoter under stress. As the most ancient anti-stress defense system, HSP70 promotes tumor cell survival by interacting with multiple points in apoptotic signaling pathways. It functions as a cellular lifeguard with anti-apoptotic effects, modulates intrinsic and extrinsic apoptotic signaling, and regulates caspase-independent apoptosis in cancer cells. Due to its anti-cancer and immunogenic properties, HSP70 inhibitors are being sought, though preclinical and clinical evaluations lag behind Hsp90 inhibitors currently in phase II/III trials. Extracellular HSP70 is immunogenic, acting as an adjuvant that, combined with chaperones, may aid vaccine production and cancer treatment. Elevated extracellular HSP70 indicates poor cancer prognosis. In syngeneic mice, HSP70 inhibition or depletion induces tumor regression, anti-tumor immune responses, and target cell apoptosis. HSP70 is an essential chaperone class that maintains proteostasis by interacting with client proteins, playing key roles in stress survival and thermotolerance development. Its function relies on a two-domain structure comprising an N-terminal nucleotide-binding domain (NBD) and a substrate-binding domain (SBD), with substrate affinity regulated by ATP hydrolysis and ADP release cycles, acting as a nucleotide-regulated clamp for transiently binding misfolded proteins.

P34932

Predicted band size: 94 kDa; Observed band size: 110 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;FC: 1/50-1/100

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human HSPA4