Hsp70 1B Antibody (YA358)

Cat. No.: HY-P80713: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Hsp70 1B Antibody (YA358) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp70 1B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Hsp70 1B Antibody (YA358) Featured Recommendations:

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
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Description	Hsp70 1B Antibody (YA358) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp70 1B.
Background	Hsp70 1B: Enables protein folding chaperone. Involved in protein folding. Acts upstream of or within binding activity of sperm to zona pellucida; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; and response to heat. Located in cell body and mitochondrion. Part of zona pellucida receptor complex. Is expressed in early conceptus; embryo; and humerus. Human ortholog(s) of this gene implicated in several diseases, including brain ischemia; obesity; toxic shock syndrome; type 2 diabetes mellitus; and urinary tract infection. Orthologous to human HSPA1B (heat shock protein family A (Hsp70) member 1B). [provided by Alliance of Genome Resources, Apr 2022]
Tag	Free
Gene ID	15511 [NCBI]
SwissProt ID	P17879
Synonyms	Hsp70; hsp68; HSP70B1; Hsp70-1; Hsp70.1
Molecular Weight	Predicted band size: 70 kDa; Observed band size: 70 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3102169
Research Field	Signal Transduction
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:20 Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp70 1B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp70 1B Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application	WB, IHC-P, IP
Appearance	Liquid
Formulation	Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Mouse Hsp70.The exact sequence is proprietary to MCE.
Database	Entrez Gene: 15511 Human ; 3303 Human ; 193740 Mouse ; 3304 Mouse ; 24472 Rat 294254 SwissProt: P0DMV8 Human ; P0DMV9 Human ; P17879 Mouse ; Q61696 Mouse ; P0DMW0 Rat P0DMW1 OMIM: 140550 Human
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Hsp70 1B Antibody (YA358) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Hsp70 1B Antibody (YA358)

Hsp70 1B Antibody (YA358) Featured Recommendations:

Biological Activity

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