HMGB1 Antibody (YA6085)

HMGB1 Antibody (YA6085) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGB1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

HMGB1 is a multifunctional redox-sensitive protein with diverse roles in various cellular compartments. In the nucleus, it acts as a major chromatin-associated non-histone protein and serves as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. In the cytoplasm, it functions as a sensor and/or chaperone for immunogenic nucleic acids, implicating TLR9-mediated immune response activation and mediating autophagy. As a danger-associated molecular pattern (DAMP) molecule, HMGB1 amplifies immune responses during tissue injury. When released into the extracellular environment, it binds to DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS), and lipoteichoic acid (LTA), activating cells through multiple surface receptors. Fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. It exhibits proangiogenic activity (by similar) and may be involved in platelet activation (by similar). It binds phosphatidylserine and phosphatidylethanolamide (by similar) and mediates neuronal outgrowth signaling via RAGE (by similar). It may also play a role in the accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP.

P09429

Predicted band size: 25 kDa; Observed band size: 25 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:2000-1:10000; WB: 1:5000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG