HLA-F Antibody (YA3943)

HLA-F Antibody (YA3943) is a mouse-derived and non-conjugated IgG2b antibody, targeting to HLA-F. It can be applicated for WB, FC, ELISA assays, in the background of human, mouse, rat.

HLA-F is a non-classical major histocompatibility class Ib molecule implicated in immune surveillance, immune tolerance, and inflammation. It functions in two forms: as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M), and as an open conformer (OC) lacking both peptide and B2M (peptide-free OC). In complex with B2M, HLA-F presents non-canonical self-peptides with post-translational modifications, particularly phosphorylated self-peptides. The peptide-bound HLA-F-B2M acts as a ligand for the inhibitory receptor LILRB1, playing a key role in maternal-fetal tolerance, while the peptide-free OC serves as a ligand for KIR3DS1 and KIR3DL2 receptors. Interaction with the activating KIR3DS1 receptor on NK cells triggers degranulation and antiviral cytokine production, whereas binding to KIR3DL2 inhibits effector functions of NK and T cells. HLA-F may also interact with other MHC class I OCs to cross-present exogenous viral, tumor, or minor histocompatibility antigens to cytotoxic CD8+ T cells, inducing effector and memory responses. Additionally, it may contribute to inflammatory responses in the peripheral nervous system and, via KIR3DL2 interaction, protect motor neurons from astrocyte-induced toxicity.

P30511

Predicted band size: 39 kDa; Observed band size: 40-50 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat

WB: 1/500-1/2000; FC: 1/200-1/400; ELISA: 1/10000

WB, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.