HDAC10 Antibody (YA394)

HDAC10 Antibody (YA394) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC10.

HDAC10 is a polyamine deacetylase (PDAC) that preferentially acts on N(8)-acetylspermidine, as well as acetylcadaverine and acetylputrescine. It exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low or negligible activity toward N(1)-acetylspermidine. In vitro studies have shown histone deacetylase activity and involvement in MSH2 deacetylation, though the physiological relevance of these activities remains unclear and may be minimal. HDAC10 may play a role in promoting late-stage autophagy (e.g., autophagosome-lysosome fusion and/or lysosomal exocytosis) in neuroblastoma cells, as well as in homologous recombination and DNA mismatch repair. by similar: HDAC10 has also been implicated in autophagy and DNA repair processes.

Free

Q969S8

Predicted band size: 71 kDa;Observed band size: 75 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102095

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1,000 ;ICC: 1:50-1:200 ;IHC:1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 1) and M-intestine (lane 2) using HDAC10 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human HDAC10.AA range:339-388.