GM130 Antibody (YA6118)

GM130 Antibody (YA6118) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GM130. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

GM130 is a peripheral membrane component of the cis-Golgi stack that functions as a membrane skeleton to maintain the structure of the Golgi apparatus and as a vesicle tether facilitating vesicle fusion to the Golgi membrane (Probable). It is required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, it participates in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and interconnected structure of the Golgi apparatus. It plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing COPI vesicle tethering and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). GM130 also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). It promotes mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, recruiting importin-alpha to Golgi membranes and liberating TPX2. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Nascent microtubules are further captured by GOLGA2, linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). It likely regulates meiotic spindle pole assembly via the same mechanism (By similarity). Additionally, it regulates centrosome organization (PubMed:18045989, PubMed:19109421) and is required for Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).

Q08379

Predicted band size: 113 kDa; Observed band size: 130 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG