2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. GCLC Antibody (YA2898)

GCLC Antibody (YA2898) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCLC.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

GCLC Antibody (YA2898) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCLC.
Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The locus encodes the catalytic subunit, while the regulatory subunit is derived from a different related gene located on chromosome 1p22-p21. Mutations at The locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Gene ID
SwissProt ID
Synonyms
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Molecular Weight

Predicted band size: 73 kDa; Observed band size: 73 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1000

  • Western blot analysis of extracts from MCF7 (lane 2(20μg)),A549 (lane 3(20μg)),C6 (lane 4(20μg)) ,Raw264.7 (lane 5(20μg)) and Jurkat (lane 6(20μg)) using GCLC Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Recombinant protein of human GCLC
Database

Entrez Gene: 2729 Human ; 14629 Mouse ; 25283 Rat

SwissProt: P48506 Human ; P97494 Mouse ; P19468 Rat

OMIM: 230450 Human

Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

GCLC Antibody (YA2898) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
GCLC Antibody (YA2898)
Cat. No.:
HY-P83153
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