GBA Antibody (YA3257)

GBA Antibody (YA3257) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3257), targeting GBA, with a predicted molecular weight of 60 kDa (observed band size: 60 kDa). GBA Antibody (YA3257) can be used for WB, IHC-P experiment in human, rat background.

GBA is a glucosylceramidase that catalyzes the hydrolysis of glucosylceramides/GlcCers (e.g., beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (e.g., N-acylsphing-4-enine) and glucose within the lysosomal compartment (PubMed:15916907, PubMed:24211208, PubMed:32144204, PubMed:9201993). It plays a central role in complex lipid degradation and cellular membrane turnover (PubMed:27378698) and participates in the PKC-activated salvage pathway of ceramide formation through ceramide production (PubMed:19279011). GBA also catalyzes cholesterol glucosylation via a transglucosylation reaction, transferring glucose from GlcCer to cholesterol (PubMed:24211208, PubMed:26724485, PubMed:32144204). GlcCers containing mono-unsaturated fatty acids (e.g., beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred glucose donors for cholesterol glucosylation over those with saturated fatty acids (e.g., beta-D-glucosyl-N-octadecanoyl-sphing-4-enine) (PubMed:24211208). Under specific conditions, GBA may reversibly transfer glucose from cholesteryl 3-beta-D-glucoside to ceramide (Probable) (PubMed:26724485) or hydrolyze cholesteryl 3-beta-D-glucoside to yield glucose and cholesterol (PubMed:24211208, PubMed:26724485). Although with lower activity, GBA can hydrolyze galactosylceramides/GalCers (e.g., beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine) and mediate galactose transfer between GalCers and cholesterol in vitro (PubMed:32144204). Unlike GlcCer and GalCer, xylosylceramide/XylCer (e.g., beta-D-xyosyl-(1<->1')-N-acylsphing-4-enine) is a poor hydrolysis substrate but serves as an efficient xylose donor for transxylosylation to form cholesteryl 3-beta-D-xyloside (PubMed:33361282).

P04062

Predicted band size: 60 kDa; Observed band size: 60 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100

WB, IHC-P

Liquid

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human GBA