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  4. GAPDH Antibody (YA3641)

GAPDH Antibody (YA3641)

Cat. No.: HY-P83944
COA User Guide for Antibodies Technical Support

GAPDH Antibody (YA3641) is a mouse-derived and non-conjugated IgG1 antibody, targeting to GAPDH. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, rabbit, monkey.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

GAPDH Antibody (YA3641) is a mouse-derived and non-conjugated IgG1 antibody, targeting to GAPDH. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, rabbit, monkey.

Background

GAPDH protein showcases a dual functionality with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, playing integral roles in glycolysis and nuclear functions, respectively. In glycolysis, it serves as a key enzyme catalyzing the initial step by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Beyond its metabolic role, GAPDH modulates the organization and assembly of the cytoskeleton and facilitates CHP1-dependent microtubule and membrane associations by stimulating the binding of CHP1 to microtubules (By similarity). It is also a component of the GAIT complex, which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. In innate immunity, GAPDH contributes to TNF-induced NF-kappa-B activation and type I interferon production by interacting with TRAF2 and TRAF3, respectively (By similarity). Furthermore, its involvement in nuclear events, including transcription, RNA transport, DNA replication, and apoptosis, is likely mediated by its nitrosylase activity, leading to cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and PRKDC (By similarity). The multifaceted functions of GAPDH underscore its versatile and critical roles in cellular processes.

Gene ID
SwissProt ID
Synonyms
G3PD; GAPD; MGC88685; Glyceraldehyde-3-phosphate dehydrogenase
Molecular Weight

Predicted band size: 36 kDa; Observed band size: 36 kDa

Purity

affinity purified.

Conjugation

Non-conjugated

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit, Monkey, Pig

Tested Reactivity

Human, Mouse, Rat, Rabbit, Monkey

Dilution Ratio

WB: 1/5000- 1/20000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

Application

WB, IHC-P, ICC/IF, FC, ELISA

Tested Applications

WB, IHC-P, ICC/IF, FC

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Immunogen

Purified recombinant fragment of human GAPDH expressed in E. Coli.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
GAPDH Antibody (YA3641)
Cat. No.:
HY-P83944
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