GAPDH Antibody (HRP) (YA874)

GAPDH Antibody (HRP) (YA874) is a HRP-conjugated, mouse-derived, anti-GAPDH (HRP) (YA874) IgG1 monoclonal antibody.

GAPDH protein showcases a dual functionality with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, playing integral roles in glycolysis and nuclear functions, respectively. In glycolysis, it serves as a key enzyme catalyzing the initial step by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Beyond its metabolic role, GAPDH modulates the organization and assembly of the cytoskeleton and facilitates CHP1-dependent microtubule and membrane associations by stimulating the binding of CHP1 to microtubules (By similarity). It is also a component of the GAIT complex, which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. In innate immunity, GAPDH contributes to TNF-induced NF-kappa-B activation and type I interferon production by interacting with TRAF2 and TRAF3, respectively (By similarity). Furthermore, its involvement in nuclear events, including transcription, RNA transport, DNA replication, and apoptosis, is likely mediated by its nitrosylase activity, leading to cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and PRKDC (By similarity). The multifaceted functions of GAPDH underscore its versatile and critical roles in cellular processes.

P04406

Predicted band size: 36 kDa; Observed band size: 36 kDa

Affinity Purified

HRP

Unmodified

AB_3103031

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:1000-1:5000; ELISA: 1:10000

• 
  Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
• 
  Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C6 (lane 4, 15 μg), and RAW264.7 (lane 5, 15 μg), using GAPDH Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:10000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

A synthetic peptide corresponding to residues near the amino terminus of human GAPDH protein.