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  4. Galectin 8 Antibody (YA2186)

Galectin 8 Antibody (YA2186)

Cat. No.: HY-P82441
COA User Guide for Antibodies Technical Support

Galectin 8 Antibody (YA2186) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Galectin 8.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

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  • Documentation

Description

Galectin 8 Antibody (YA2186) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Galectin 8.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight
Predicted band size: 36 kDa;
Observed band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Galectin 8 aa10-59.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
ALL WB FC
  • Western blot analysis of extracts from HepG2 (lane 2(20μg) , HepG2 (lane 3(40μg),using Galectin 8 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary anti-body and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Anti-body (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Flow cytometric analysis of 1X10^6 Hela cells labeling Galectin 8 Antibody (YA2186) (HY-P82441, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

RRID
Database
Research Field

Cancer

Synonyms
LGALS8; Galectin-8g; Galectin-8; Po66-CBP; PCTA-1; Gal-8; Galectin 8; PCTA1
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Galectin 8 Antibody (YA2186)
Cat. No.:
HY-P82441
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