G3BP1 Antibody (YA5543)

G3BP1 Antibody (YA5543) is a mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to G3BP1. It can be applicated for WB, ICC/IF, ELISA assays, in the background of human, mouse, rat.

G3BP is a multifunctional protein involved in processes such as stress granule formation and innate immunity. It plays an essential role in stress granule formation, which are membraneless compartments storing mRNAs and proteins during stress responses. G3BP promotes stress granule assembly through liquid-liquid phase separation (LLPS), acting as a molecular switch that triggers RNA-dependent LLPS in response to increased intracellular free RNA concentrations. Additionally, G3BP exhibits ATP- and magnesium-dependent helicase activity, unwinding DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency while moving unidirectionally along single-stranded DNA. In innate immunity, G3BP enhances CGAS and RIGI activity, promoting the DNA-triggered cGAS/STING pathway and RIGI-mediated type I interferon production. In vitro, G3BP may also function as a phosphorylation-dependent sequence-specific endoribonuclease, preferentially cleaving MYC mRNA at the 3'-UTR. by similar: G3BP is implicated in cGAS condensation, likely associated with the formation of membrane-less organelles.

Q13283

Predicted band size: 52 kDa; Observed band size: 68 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Synthesized peptide derived from human protein. AA range: 200-300