Fyn Antibody (YA5237)

Fyn Antibody (YA5237) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Fyn. It can be applicated for WB, ICC assays, in the background of human, monkey, rat, mouse.

Fyn is a non-receptor tyrosine-protein kinase involved in regulating diverse biological processes such as cell growth/survival, adhesion, integrin-mediated signaling, cytoskeletal remodeling, motility, immune response, and axon guidance. Inactive Fyn is phosphorylated at its C-terminal tail; upon PKA activation, it associates with PTK2/FAK1 to facilitate FAK1 phosphorylation and focal adhesion targeting. It modulates adhesion/motility via CTNNB1 (β-catenin) and CTNND1 (δ-catenin) phosphorylation, and drives cytoskeletal remodeling by phosphorylating WAS, MAP2, and MAPT. Fyn promotes cell survival by inhibiting AGAP2/PIKE-A cleavage and maintains glomerular slit diaphragm integrity via NPHS1/KIRREL1/TRPC6 phosphorylation. In neural contexts, it phosphorylates DPYSL2, ARHGAP32, and SNCA. Fyn also mediates TCR-induced T-cell proliferation/differentiation by phosphorylating PTK2B/PYK2 and negatively regulates TCR signaling through PAG1-CSK interactions. Similar roles include: DAB1 phosphorylation in reelin signaling (by similar); CLNK phosphorylation in IgE receptor-activated mast cells (by similar); and CD244-dependent NK cell activation.

P06241

Observed band size: 59 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Monkey, Rat, Mouse

WB: 1:500; ICC: 1:50

WB, ICC

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human Fyn protein fragments expressed in E.coli.