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  4. FOXP3 Antibody (YA425)

FOXP3 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 47 kDa, targeting to FOXP3. It can be used for WB,ICC,FC assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

FOXP3 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 47 kDa, targeting to FOXP3. It can be used for WB,ICC,FC assays with tag free, in the background of Human.

Tag

Free

Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 47 kDa

Purity

Protein A affinity purified.

Subcellular Location

Nucleus, Cytoplasm.

Conjugation

Non-conjugated

Modification

Unmodified

RRID
Research Field

Cell Biology

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human

Dilution Ratio

WB: 1:500-1:2,000 ;ICC: 1:50-1:100 ;FC: 1:50-1:100

  • Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ICC/IF, FC

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human FOXP3.AA range:250-431.

Database
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
FOXP3 Antibody (YA425)
Cat. No.:
HY-P80135
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