FOXO1A Antibody (YA6280)

FOXO1A Antibody (YA6280) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXO1A. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse.

FoxO1 is a transcription factor that serves as the primary target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. It binds to the insulin response element (IRE) and Daf-16 family binding element (DBE), with its activity suppressed by insulin. FoxO1 is a key regulator of redox balance and osteoblast numbers, controlling bone mass (By similarity). It orchestrates the endocrine function of the skeleton in glucose metabolism regulation (By similarity). Under conditions of low lipid availability, FoxO1 translocates to the nucleus and promotes SOX9 expression, driving chondrogenic commitment while suppressing fatty acid oxidation (By similarity). FoxO1 synergizes with ATF4 to inhibit osteocalcin (BGLAP) activity, elevating blood glucose levels and triggering glucose intolerance and insulin resistance (By similarity). It also suppresses the transcriptional activity of RUNX2 (By similarity). In pancreatic beta cells, FoxO1 inhibits glucose sensing by repressing PDX1 expression (By similarity). In hepatocytes, FoxO1 promotes gluconeogenesis by co-activating genes such as IGFBP1, G6PC1, and PCK1 with PPARGC1A and CEBPA (By similarity). Additionally, it directly enhances PPARGC1A and G6PC1 expression to drive gluconeogenesis. FoxO1 is a critical regulator of cell death downstream of CDK1, PKB/AKT1, and STK4/MST1 (By similarity). It induces neural cell death and mediates insulin action in adipose tissue (By similarity). During preadipocyte differentiation, FoxO1 regulates adipogenic genes like PPARG and modulates adipocyte size and adipose-specific gene expression in response to excessive calorie intake (By similarity). It also governs GADD45A transcriptional activity and repairs nitric oxide-damaged DNA in beta cells (By similarity). In cardiomyocytes, FoxO1 mediates MLIP's role in hypertrophy and cardiac remodeling (By similarity). In cardiac smooth muscle cells, it promotes apoptosis by activating pro-apoptotic genes (By similarity). In endothelial cells (ECs), FoxO1 regulates viability and apoptosis via CCL2 and BCL2L11 transcription, which are involved in EC chemotaxis and apoptosis (By similarity).

Q12778

Predicted band size: 75 kDa; Observed band size: 75 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse,

IHC: 1:400-1000; WB: 1:1000-5000; ICC/IF: 1:200-1000; ELISA: 1:5000-20000; IP: 1:50-200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG