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  2. Primary Antibodies
  3. Monoclonal Antibodies
  4. FOXO1(C-term) Antibody (YA5217)

FOXO1(C-term) Antibody (YA5217)

Cat. No.: HY-P85525
User Guide for Antibodies Technical Support

FOXO1(C-term) Antibody (YA5217) is a mouse-derived and non-conjugated monoclonal antibody, targeting to FOXO1 (C-term). It can be applicated for WB assays, in the background of human.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

FOXO1(C-term) Antibody (YA5217) is a mouse-derived and non-conjugated monoclonal antibody, targeting to FOXO1 (C-term). It can be applicated for WB assays, in the background of human.

Background

FoxO1 is a transcription factor that serves as the primary target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. It binds to the insulin response element (IRE) and Daf-16 family binding element (DBE), with its activity suppressed by insulin. FoxO1 is a key regulator of redox balance and osteoblast numbers, controlling bone mass (By similarity). It orchestrates the endocrine function of the skeleton in glucose metabolism regulation (By similarity). Under conditions of low lipid availability, FoxO1 translocates to the nucleus and promotes SOX9 expression, driving chondrogenic commitment while suppressing fatty acid oxidation (By similarity). FoxO1 synergizes with ATF4 to inhibit osteocalcin (BGLAP) activity, elevating blood glucose levels and triggering glucose intolerance and insulin resistance (By similarity). It also suppresses the transcriptional activity of RUNX2 (By similarity). In pancreatic beta cells, FoxO1 inhibits glucose sensing by repressing PDX1 expression (By similarity). In hepatocytes, FoxO1 promotes gluconeogenesis by co-activating genes such as IGFBP1, G6PC1, and PCK1 with PPARGC1A and CEBPA (By similarity). Additionally, it directly enhances PPARGC1A and G6PC1 expression to drive gluconeogenesis. FoxO1 is a critical regulator of cell death downstream of CDK1, PKB/AKT1, and STK4/MST1 (By similarity). It induces neural cell death and mediates insulin action in adipose tissue (By similarity). During preadipocyte differentiation, FoxO1 regulates adipogenic genes like PPARG and modulates adipocyte size and adipose-specific gene expression in response to excessive calorie intake (By similarity). It also governs GADD45A transcriptional activity and repairs nitric oxide-damaged DNA in beta cells (By similarity). In cardiomyocytes, FoxO1 mediates MLIP's role in hypertrophy and cardiac remodeling (By similarity). In cardiac smooth muscle cells, it promotes apoptosis by activating pro-apoptotic genes (By similarity). In endothelial cells (ECs), FoxO1 regulates viability and apoptosis via CCL2 and BCL2L11 transcription, which are involved in EC chemotaxis and apoptosis (By similarity).

Gene ID
SwissProt ID
Synonyms
FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead; Drosophila; homolog 1; rhabdomyosarcoma; Forkhead; Drosophila; homolog 1; rhabdomyosarcoma; Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A; OTTHUMP00000018301.
Molecular Weight

Observed band size: 70 kDa

Purity

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Clonality

Monoclonal

Host

Mouse

Reactivity

Human

Dilution Ratio

WB: 1:1000

Application

WB

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Immunogen

Purified recombinant human FOXO1(C-terminus) protein fragments expressed in E.coli.

Documentation

FOXO1(C-term) Antibody (YA5217) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FOXO1(C-term) Antibody (YA5217)
Cat. No.:
HY-P85525
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