Flk-1/VEGFR2 Antibody (YA4860)

Flk-1/VEGFR2 Antibody (YA4860) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Flk-1/VEGFR2. It can be applicated for WB, ChIP, ICC/IF, FC, ELISA assays, in the background of human, fish.

Flk-1/VEGFR2 is a tyrosine-protein kinase that functions as a cell-surface receptor for VEGFA, VEGFC, and VEGFD. It plays a pivotal role in regulating angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis by promoting endothelial cell proliferation, survival, migration, and differentiation, as well as reorganizing the actin cytoskeleton. Isoforms lacking a transmembrane domain (e.g., isoform 2 and isoform 3) may act as decoy receptors for VEGFA, VEGFC, and/or VEGFD. Specifically, isoform 2 negatively regulates VEGFA- and VEGFC-mediated lymphangiogenesis by sequestering free ligands and blocking their binding to FLT4. The receptor modulates FLT1 and FLT4 signaling through heterodimer formation. Ligand binding to isoform 1 triggers multiple signaling cascades, including PLCG1 activation (generating diacylglycerol and inositol 1,4,5-trisphosphate), protein kinase C activation, MAPK1/ERK2, MAPK3/ERK1, MAP kinase pathway, and AKT1 pathway. It also mediates phosphorylation of PIK3R1 (regulatory subunit of phosphatidylinositol 3-kinase), PTK2/FAK1 activation, and VEGFA-induced NOS2/NOS3 expression to stimulate nitric oxide (NO) production in endothelial cells. Known phosphorylation substrates include PLCG1, FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1, and SRC.

P35968

Predicted band size: 152 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Fish

WB: 1:500-1:2000; ICC/IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

WB, ChIP, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant extracellular fragment of human Flk-1 (aa20-764) fused with hIgGFc tag expressed in HEK293 cells.