FEN-1 Antibody (YA5295)

FEN-1 Antibody (YA5295) is a mouse-derived and non-conjugated monoclonal antibody, targeting to FEN-1. It can be applicated for WB, IP assays, in the background of human, mouse, rat.

FEN-1 is a structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, it cleaves the 5'-overhanging flap structure generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. FEN-1 enters the flap from the 5'-end and tracks to cleave the flap base, leaving a nick for ligation. It also participates in the long patch base excision repair (LP-BER) pathway by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. As a genome stabilization factor, FEN-1 prevents flaps from equilibrating into structures that lead to duplications and deletions. Additionally, it exhibits 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and RNase H activity. FEN-1 is further involved in the replication and repair of rDNA and mitochondrial DNA.

P39748

Observed band size: 45 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:1000

WB, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human FEN-1 protein fragments expressed in E.coli.