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  5. FADD Antibody (YA2793)

FADD Antibody (YA2793) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2793), targeting FADD, with a predicted molecular weight of 23 kDa (observed band size: 28 kDa). FADD Antibody (YA2793) can be used for WB, IHC-P experiment in human, mouse background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

FADD Antibody (YA2793) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2793), targeting FADD, with a predicted molecular weight of 23 kDa (observed band size: 28 kDa). FADD Antibody (YA2793) can be used for WB, IHC-P experiment in human, mouse background.
Background

FADD (Fas-associated protein with death domain) is an apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting complex, termed the death-inducing signaling complex (DISC), mediates proteolytic activation of CASP8. Active CASP8 then initiates a downstream cascade of caspases to execute apoptosis. Additionally, FADD is involved in interferon-mediated antiviral immune responses by positively regulating interferon signaling pathways.
Gene ID
SwissProt ID
Synonyms
Mort1/FADD
Molecular Weight

Predicted band size: 23 kDa; Observed band size: 28 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100

  • Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IHC-P
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of mouse FADD
Database

Entrez Gene: 8772 Human ; 14082 Mouse ;

SwissProt: Q13158 Human ; Q61160 Mouse ;

OMIM: 613759 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

FADD Antibody (YA2793) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
FADD Antibody (YA2793)
Cat. No.:
HY-P83048
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