ERK2 Antibody (YA6346)

ERK2 Antibody (YA6346) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK1/2. It can be applicated for WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, FC assays, in the background of human, mouse, rat.

ERK2, a serine/threonine kinase and indispensable component of the MAP kinase signal transduction pathway, collaborates with MAPK1/ERK2 in orchestrating the intricate MAPK/ERK cascade. Activated by stimuli such as KIT and KITLG/SCF, this pathway assumes a central role in diverse cellular functions, including cell growth, adhesion, survival, and differentiation, achieved through the regulation of transcription, translation, and cytoskeletal rearrangements. ERK2 further contributes to the initiation and regulation of meiosis, mitosis, and postmitotic functions by phosphorylating numerous transcription factors. With approximately 160 substrates identified, ERK2 impacts cellular processes in the nucleus, cytosol, and various cellular organelles, influencing transcription, translation, mitosis, and apoptosis. Additionally, the MAPK/ERK cascade, facilitated by ERK2, is implicated in endosomal dynamics, lysosome processing, endosome cycling through the perinuclear recycling compartment (PNRC), and Golgi apparatus fragmentation during mitosis. Substrates of ERK2 span transcription factors, cytoskeletal elements, regulators of apoptosis, regulators of translation, and diverse signaling-related molecules, as well as protein kinases and phosphatases. Notably, ERK2 mediates phosphorylation events, such as those on TPR in response to EGF stimulation, and plays a role in the spindle assembly checkpoint. It phosphorylates PML, promoting its interaction with PIN1 and subsequent degradation, and phosphorylates CDK2AP2. Acting as a transcriptional repressor, ERK2 binds to specific consensus sequences and represses the expression of interferon gamma-induced genes, demonstrating its multifaceted regulatory role beyond its kinase activity.

P28482

Predicted band size: 42/44 kDa

affinity purified.

Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization.

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-2000; IHC-P: 1:100-500; IHC-F: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; Flow-Cyt: 1ug/Test

WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, FC

Liquid

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

KLH conjugated synthetic peptide derived from human ERK1/2