eRF3a Antibody

Cat. No.: HY-P83766: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

eRF3a Antibody is a rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a. It can be applicated for WB, IHC-P assays, in the background of human, mouse, rat.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
Technical Parameters
Properties
Documentation

Description	eRF3a Antibody is a rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a. It can be applicated for WB, IHC-P assays, in the background of human, mouse, rat.
Background	eRF3a is the GTPase component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination in response to stop codons UAA, UAG, and UGA. GSPT1/ERF3A delivers ETF1/ERF1 to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site, and GTP hydrolysis by GSPT1/ERF3A induces a conformational change leading to its dissociation, allowing ETF1/ERF1 to fully occupy the A-site. Additionally, eRF3a is part of the transient SURF complex that recruits UPF1 to stalled ribosomes during nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It is also required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) in viral and cellular mRNAs. by similar: Its functions are supported by studies on complex binding, conformational dynamics, and roles in NMD and frameshifting regulation.
Gene ID	2935 [NCBI]
SwissProt ID	P15170
Synonyms	Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; GSPT1; ERF3A
Molecular Weight	Predicted band size: 55 kDa; Observed band size: 80 kDa
Purity	Affinity Purified
Conjugation	Non-conjugated
Modification	Unmodified
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1/500-1/1000; IHC: 1/50-1/200
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Human eRF3a.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (204 KB) User Guide for Antibodies (1077 KB)

eRF3a Antibody Related Classifications

Primary Antibodies

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eRF3a Antibody

Biological Activity

Technical Parameters

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Documentation

eRF3a Antibody Related Classifications

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