eRF3a Antibody

Cat. No.: HY-P83766: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

eRF3a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

eRF3a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P15170

Gene ID

2935 [NCBI]

Immunogen

Synthetic peptide corresponding to Human eRF3a.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC	1:50-1:200

Appearance

Liquid

Formulation

Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and 293 (lane 4, 15 μg) using eRF3a Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Synonyms

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; GSPT1; ERF3A

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)

eRF3a Antibody Related Classifications

Primary Antibodies

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eRF3a Antibody

Product Detail

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Background

Documentation

eRF3a Antibody Related Classifications

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