Neutrophil Elastase Antibody (YA3701)

Neutrophil Elastase Antibody (YA3701) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ELANE. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, monkey.

ELANE, a serine protease, plays a crucial role in modulating the functions of natural killer cells, monocytes, and granulocytes. It acts by inhibiting C5a-dependent neutrophil enzyme release and chemotaxis, highlighting its regulatory role in immune responses. Additionally, ELANE participates in the inhibition of pyroptosis by promoting the cleavage of GSDMB, showcasing its involvement in the regulation of programmed cell death. Notably, ELANE exhibits bactericidal activity by effectively killing E. coli in vitro, while sparing S. aureus. This antimicrobial effect is attributed to its ability to digest outer membrane protein A (ompA) in E. coli and K. pneumoniae, underscoring its role in bacterial defense mechanisms.

P08246

Predicted band size: 29 kDa; Observed band size: 29 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli.