EIF5 Antibody (YA4096)

EIF5 Antibody (YA4096) is a mouse-derived and non-conjugated IgG2a antibody, targeting to EIF5. It can be applicated for IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

EIF5 serves as a crucial component of the 43S pre-initiation complex (43S PIC), participating in the initiation of protein synthesis. Within this complex, EIF5 plays a key role in mRNA scanning, start codon recognition, and GTP hydrolysis. Acting as a GTPase-activating protein, EIF5 promotes the hydrolysis of GTP by eIF2G (EIF2S3). During the scanning process, EIF5 interacts with both EIF1 and EIF1A, contributing to the maintenance of EIF1 within the open 43S PIC. Upon recognition of the start codon, EIF5 induces eIF2G (EIF2S3) to hydrolyze GTP and initiates a conformational change in the PIC to a closed state. This change enhances the affinity of EIF5-CTD for EIF2-beta (EIF2S2), leading to the release of EIF1 from the PIC. Finally, EIF5 stabilizes the PIC in its closed conformation. EIF5 interacts with various components of the translation machinery, including EIF1A, EIF2-beta (EIF2S2), and EIF5B, as well as with FMR1 isoform 6 in a RNA-dependent manner, highlighting its multifaceted involvement in protein synthesis initiation.

P55010

Predicted band size: 50 kDa; Observed band size: 50-60 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; IF: 1/50-1/250 FC: 1/200-1/400; ELISA: 1/10000

IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.