EHMT2 Antibody (YA4431)

EHMT2 Antibody (YA4431) is a mouse-derived and non-conjugated IgG1 antibody, targeting to EHMT2. It can be applicated for IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

EHMT2 is a histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2) in euchromatin. H3K9me serves as a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Additionally, EHMT2 mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) during G1 phase, promoting interaction between histone H3 and PCNA and regulating DNA replication. It also weakly methylates 'Lys-27' of histone H3 (H3K27me). EHMT2 is required for DNA methylation, though its histone methyltransferase activity is not necessary for this process, indicating independent functions. It is likely targeted to histone H3 by DNA-binding proteins such as E2F6, MGA, MAX, and/or DP1 and may methylate histone H1. Beyond histone methylation, EHMT2 methylates non-histone proteins, including dimethylation of 'Lys-373' of p53/TP53, and methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12, and itself.

Q96KQ7

Predicted band size: 132 kDa; Observed band size: 170 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli.