E2F-1 Antibody (YA4153)

E2F-1 Antibody (YA4153) is a mouse-derived and non-conjugated IgG1 antibody, targeting to E2F1. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse.

E2F1 is a transcription activator that cooperatively binds DNA with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3', located in the promoter regions of genes involved in cell cycle regulation or DNA replication. The DRTF1/E2F complex controls cell cycle progression from G1 to S phase. E2F1 preferentially binds RB1 in a cell cycle-dependent manner and can mediate both cell proliferation and TP53/p53-dependent apoptosis. It blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. E2F1 directly activates PEG10 transcription and positively regulates RRP1B transcription. by similar: E2F1 shares some functions with other E2F family members, such as forming complexes with DP proteins to regulate the cell cycle.

Q01094

Predicted band size: 47 kDa; Observed band size: 47 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.