E2F-1 Antibody (YA5305)

E2F-1 Antibody (YA5305) is a mouse-derived and non-conjugated monoclonal antibody, targeting to E2F-1. It can be applicated for WB, ICC/IF, IP assays, in the background of human, rat.

E2F1 is a transcription activator that cooperatively binds DNA with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3', located in the promoter regions of genes involved in cell cycle regulation or DNA replication. The DRTF1/E2F complex controls cell cycle progression from G1 to S phase. E2F1 preferentially binds RB1 in a cell cycle-dependent manner and can mediate both cell proliferation and TP53/p53-dependent apoptosis. It blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. E2F1 directly activates PEG10 transcription and positively regulates RRP1B transcription. by similar: E2F1 shares some functions with other E2F family members, such as forming complexes with DP proteins to regulate the cell cycle.

Q01094

Observed band size: 60 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat

WB: 1:500; ICC: 1:100; ICC/IF: 1:50-200

WB, ICC/IF, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human E2F-1 protein fragments expressed in E.coli.