Dynamin I Antibody (YA4817)

Dynamin I Antibody (YA4817) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Dynamin I. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

Dynamin 1 is a GTPase that catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission, participating in various forms of endocytosis, including clathrin-mediated endocytosis, synaptic vesicle endocytosis, and rapid endocytosis (RE). It associates with membranes through lipid binding and self-assembles into rings and interconnected stacks, forming a helical polymer around the vesicle membrane to constrict invaginated coated pits. The self-assembly of the helical polymer narrows membrane tubules until GTP hydrolysis is triggered. Depending on membrane curvature, detachment from the polymer after GTP hydrolysis can lead to spontaneous hemifission followed by complete fission. In non-neuronal cells, Dynamin 1 may regulate early stages of clathrin-mediated endocytosis through activation by dephosphorylation downstream of EGFR signaling. At hippocampal synapses, it controls vesicle size during membrane pit formation (by similar). It also mediates plastic adaptation of synaptic vesicle recycling to high activity levels (by similar) and facilitates rapid endocytosis, a Ca²⁺-dependent, clathrin- and K(+)-independent process in chromaffin cells (by similar). As a microtubule-associated force-producing protein, it bundles microtubules and binds/hydrolyzes GTP (by similar). Through interaction with DNAJC6, it acts during early steps of clathrin-coated vesicle (CCV) formation.

Q05193

Predicted band size: 97 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human Dynamin I expressed in E. Coli.