Drosha Antibody (YA472)

Drosha Antibody (YA472) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Drosha.

Drosha is a double-stranded (ds) RNA-specific endoribonuclease (Ribonuclease III) involved in the initial step of microRNA (miRNA) biogenesis. As a core component of the microprocessor complex, Drosha processes primary miRNA transcripts (pri-miRNAs) in the nucleus to release precursor miRNAs (pre-miRNAs). Within this complex, Drosha cleaves both the 3' and 5' strands of pri-miRNA stem-loops (11 bp from the dsRNA-ssRNA junction) to generate hairpin-shaped pre-miRNAs, which are subsequently processed by cytoplasmic DICER into mature miRNAs. It also participates in pre-rRNA processing, specifically cleaving dsRNA while avoiding ssRNA, and contributes to GW body formation. By similarity, Drosha plays a role in growth homeostasis regulation through autophagy responses in motor neurons.

Free

Q9NRR4

Predicted band size: 159 kDa; Observed band size: 159 kDa

Protein A affinity purified.

Nucleus.

Non-conjugated

Unmodified

AB_3102826

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; ICC: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg), SiHa (lane 4(20μg) and SiHa (lane 5(40μg) using Drosha (HY-P80110) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded human Liver cancer tissue using Drosha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Drosha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human kidney tissue using Drosha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Drosha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80110, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

WB, ICC/IF, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Drosha.AA range:30-79.