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  5. Drebrin Antibody (YA3318)

Drebrin Antibody (YA3318)

Cat. No.: HY-P83573
COA
SDS
User Guide for Antibodies Technical Support

Drebrin Antibody (YA3318) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3318), targeting Drebrin, with a predicted molecular weight of 71 kDa (observed band size: 120 kDa). Drebrin Antibody (YA3318) can be used for WB, ICC/IF, IP experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Drebrin Antibody (YA3318) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3318), targeting Drebrin, with a predicted molecular weight of 71 kDa (observed band size: 120 kDa). Drebrin Antibody (YA3318) can be used for WB, ICC/IF, IP experiment in human, mouse, rat background.
Background

Drebrin is an actin cytoskeleton-organizing protein that plays a role in the formation of cell projections. It is required for actin polymerization at immunological synapses (IS) and for the recruitment of the chemokine receptor CXCR4 to IS. Additionally, Drebrin is involved in dendritic spine morphogenesis and organization, including the localization of the dopamine receptor DRD1 to dendritic spines (by similarity). It also participates in memory-related synaptic plasticity in the hippocampus (by similarity).
Gene ID
SwissProt ID
Synonyms
D0S117E; DBN1; Drebrin 1; Drebrin; Drebrin E2; Drebrin1; DrebrinE
Molecular Weight

Predicted band size: 71 kDa; Observed band size: 120 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Neuroscience
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IF: 1/50-1/200;IP: 1/50

  • Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using Drebrin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, ICC/IF, IP
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human Drebrin
Database

Entrez Gene: 1627 Human ; 56320 Mouse ; 81653 Rat

SwissProt: Q16643 Human ; Q9QXS6 Mouse ; Q07266 Rat

OMIM: 104300 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

Drebrin Antibody (YA3318) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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