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  4. DHFR Antibody (YA3335)

DHFR Antibody (YA3335) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3335), targeting DHFR, with a predicted molecular weight of 21 kDa (observed band size: 21 kDa). DHFR Antibody (YA3335) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

DHFR Antibody (YA3335) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3335), targeting DHFR, with a predicted molecular weight of 21 kDa (observed band size: 21 kDa). DHFR Antibody (YA3335) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

Background

Dihydrofolate reductase (DHFR) stands as a pivotal enzyme in folate metabolism, playing a crucial role in multiple essential pathways. It contributes significantly to the de novo mitochondrial thymidylate biosynthesis pathway, catalyzing reactions vital for de novo glycine and purine synthesis, as well as the synthesis of DNA precursors. Beyond its catalytic functions, DHFR exhibits the ability to bind its own mRNA and that of DHFR2, thereby suggesting a regulatory role in the control of its own expression and potentially influencing related cellular processes.

Gene ID
SwissProt ID
Synonyms
DHFR; DHFRP1; Dihydrofolate reductase; DYR
Molecular Weight

Predicted band size: 21 kDa; Observed band size: 21 kDa

Purity

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

Application

WB, IHC-P, ICC/IF

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

A synthesized peptide derived from human DHFR

Database
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DHFR Antibody (YA3335)
Cat. No.:
HY-P83590
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