DFNA5/GSDME Antibody (YA6002)

DFNA5/GSDME Antibody (YA6002) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DFNA5. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

DFNA5 is a precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. Its N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores upon cleavage, triggering pyroptosis. The protein can be cleaved by CASP3 or granzyme B (GZMB), promoting the switch from apoptosis to pyroptosis or granzyme-mediated pyroptosis, respectively. After cleavage, DFNA5 translocates to the plasma membrane, forms pores (10-15 nm in diameter), and releases mature interleukins (e.g., IL1B and IL16) to induce pyroptosis. It also binds to bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. CASP3-mediated cleavage enables DFNA5 to convert TNF- or chemotherapy-induced apoptosis into pyroptosis. Additionally, DFNA5 mediates secondary necrosis downstream of mitochondrial apoptosis and viral infections, exhibits bactericidal activity, and suppresses tumors via GZMB-triggered pyroptosis in NK cell targets. Similar roles include: involvement in p53/TP53-regulated DNA damage response. Microbial infection-related function: DFNA5 promotes maternal placental pyroptosis during Zika virus infection, contributing to adverse fetal outcomes.

O60443

Predicted band size: 55 kDa; Observed band size: 55 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG