DDX58 Antibody (YA3574)

DDX58 Antibody (YA3574) is a mouse-derived and non-conjugated IgM antibody, targeting to DDX58. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.

DDX58 protein serves as an innate immune receptor crucial for detecting cytoplasmic viral nucleic acids, initiating a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines. It forms a ribonucleoprotein complex with viral RNAs, promoting homooligomerization into filaments. This oligomerization facilitates the recruitment of RNF135, an E3 ubiquitin-protein ligase, which amplifies RIG-I-mediated antiviral signaling. Upon activation, DDX58 associates with MAVS/IPS1, activating TBK1 and IKBKE kinases, leading to the phosphorylation of IRF3 and IRF7. This activation prompts the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. DDX58 recognizes various viral RNA ligands, such as 5'-triphosphorylated ssRNAs and dsRNAs, with the 5'-triphosphate moiety and blunt-end base pairing being crucial for its activity. The protein detects a broad range of RNA viruses and plays a role in antiviral signaling against dsDNA-containing viruses, such as Epstein-Barr virus. Additionally, it participates in granulocyte production, differentiation, bacterial phagocytosis, and the regulation of cell migration.

O95786

Predicted band size: 107 kDa; Observed band size: 107 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgM

Purified recombinant fragment of human DDX58 (AA: 789-925) expressed in E. Coli.