DDX58 Antibody (YA2318)

DDX58 Antibody (YA2318) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2318), targeting DDX58, with a predicted molecular weight of 107 kDa (observed band size: 107 kDa). DDX58 Antibody (YA2318) can be used for WB, IP experiment in human background.

DDX58 protein serves as an innate immune receptor crucial for detecting cytoplasmic viral nucleic acids, initiating a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines. It forms a ribonucleoprotein complex with viral RNAs, promoting homooligomerization into filaments. This oligomerization facilitates the recruitment of RNF135, an E3 ubiquitin-protein ligase, which amplifies RIG-I-mediated antiviral signaling. Upon activation, DDX58 associates with MAVS/IPS1, activating TBK1 and IKBKE kinases, leading to the phosphorylation of IRF3 and IRF7. This activation prompts the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. DDX58 recognizes various viral RNA ligands, such as 5'-triphosphorylated ssRNAs and dsRNAs, with the 5'-triphosphate moiety and blunt-end base pairing being crucial for its activity. The protein detects a broad range of RNA viruses and plays a role in antiviral signaling against dsDNA-containing viruses, such as Epstein-Barr virus. Additionally, it participates in granulocyte production, differentiation, bacterial phagocytosis, and the regulation of cell migration.

O95786

Predicted band size: 107 kDa; Observed band size: 107 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104674

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IP: 1/50

• 
  Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using DDX58 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human DDX58