DDX5 Antibody (YA3838)

DDX5 Antibody (YA3838) is a mouse-derived and non-conjugated IgG2a antibody, targeting to DDX5. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse, monkey.

DDX5 is an RNA helicase involved in the alternative regulation of pre-mRNA splicing, where its helicase activity promotes tau exon 10 inclusion in an RBM4-dependent manner. It binds to the stem-loop region downstream of exon 10 in tau pre-mRNA, with ATP hydrolysis rates highly stimulated by single-stranded RNA. Beyond splicing, DDX5 participates in transcription regulation (independent of helicase activity), acting as a coactivator for androgen receptor AR (but not ESR1) and synergizing with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity during skeletal muscle differentiation. It also serves as a coactivator for p53/TP53 in DNA damage response and apoptosis, and for RUNX2 in osteoblast differentiation. Additionally, DDX5 exhibits promoter-specific transcriptional repression likely through HDAC1 interaction, and as part of a PER complex, inhibits 3' transcriptional termination of circadian genes like PER1 and NR1D1 to modulate circadian rhythms.

P17844

Predicted band size: 69 kDa; Observed band size: 69 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. Coli.