DDX3X Antibody (YA3492)

DDX3X Antibody (YA3492) is a mouse-derived and non-conjugated IgG2a antibody, targeting to DDX3X. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey, rabbit.

DDX3 is a multifunctional ATP-dependent RNA helicase whose ATPase activity can be stimulated by various ribo- and deoxyribonucleic acids, indicating relaxed substrate specificity. It can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs and binds RNA G-quadruplex (rG4s) structures. DDX3 is involved in numerous cellular processes, including transcription regulation, translation initiation, and innate immunity. In transcription regulation, it positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-dependent manner to inhibit cell growth while repressing CDH1/E-cadherin transcription. In translation regulation, DDX3 promotes efficient translation of selected mRNAs with complex 5'-UTRs and facilitates the assembly of functional 80S ribosomes independently of its ATPase activity. In innate immunity, DDX3 acts as a viral RNA sensor, binding viral RNAs and promoting type I interferon (IFN-alpha and IFN-beta) production. Additionally, DDX3 participates in stress granule assembly, NLRP3 inflammasome regulation, and activation of the Wnt/beta-catenin signaling pathway. During viral infections, DDX3 facilitates the replication of HCV, HIV-1, ZIKV, DENV, and VEEV.

O00571

Predicted band size: 73 kDa; Observed band size: 73 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Monkey, Rabbit

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Purified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli.