Cytochrome P450 17A1 Antibody (YA5258)

Cytochrome P450 17A1 Antibody (YA5258) is a mouse-derived and non-conjugated monoclonal antibody, targeting to CYP17A1. It can be applicated for WB assays, in the background of human.

Cytochrome P450 17A1 is a cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. It catalyzes the 17-alpha hydroxylation of C21 steroids, a common step in both pathways. A second oxidative step, required for androgen synthesis, involves acyl-carbon cleavage. The 17-alpha hydroxy intermediates serve as precursors of cortisol in the adrenal glucocorticoid biosynthesis pathway. This enzyme hydroxylates steroid hormones, pregnenolone, and progesterone to form 17-alpha hydroxy metabolites, followed by C17-C20 bond cleavage to produce C19 steroids such as dehydroepiandrosterone (DHEA) and androstenedione. It also exhibits 16-alpha hydroxylase activity, catalyzing the 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by C17-C20 bond cleavage to form 16-alpha-hydroxy DHEA, and hydroxylates androgens, which is relevant for estriol synthesis. Mechanistically, it uses molecular oxygen, inserting one oxygen atom into the substrate and reducing the second to water, with electrons supplied by NADPH via cytochrome P450 reductase (CPR).

P05093

Observed band size: 60 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:1000

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human Cytochrome P450 17A1 protein fragments expressed in E.coli.