CYP7A1 Antibody (YA5117)

CYP7A1 Antibody (YA5117) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CYP7A1.

CYP7A1 is a cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). It utilizes molecular oxygen to insert one oxygen atom into a substrate while reducing the second into a water molecule, with two electrons supplied by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). As a critical regulatory enzyme in bile acid biosynthesis and cholesterol homeostasis, CYP7A1 catalyzes the hydroxylation of the 7-alpha carbon-hydrogen bond of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid synthesis. Additionally, it 7-alpha hydroxylates various oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol, and oxidizes the 7,8 double bond of 7-dehydrocholesterol and lathosterol, predominantly forming 7-keto derivatives.

P22680

Predicted band size: 58 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Golden hamster

WB: 1:1000-1:2000

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human CYP7A1 (C-terminus) protein fragments expressed in E.coli.