Cyclin T1 Antibody (YA2600)

Cyclin T1 Antibody (YA2600) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2600), targeting Cyclin T1, with a predicted molecular weight of 81 kDa (observed band size: 81 kDa). Cyclin T1 Antibody (YA2600) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, mouse, rat background.

Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also known as positive transcription elongation factor B (P-TEFb). This complex facilitates the transition from abortive to productive elongation by phosphorylating the C-terminal domain (CTD) of the large subunit of RNA polymerase II (RNA Pol II). Cyclin T1 activates the protein kinase activity of CDK9 by mediating the formation of liquid-liquid phase separation (LLPS), enhancing the binding of P-TEFb to the CTD of RNA Pol II. During HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral transcriptional activator Tat, increasing Tat's affinity for the transactivating response RNA element (TAR RNA). It serves as an essential cofactor for Tat, promoting RNA Pol II activation and enabling the transcription of viral genes.

O60563

Predicted band size: 81 kDa; Observed band size: 81 kDa

Affinity Purified

Non-conjugated

Unmodified

Microbiology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using Cyclin T1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human Cyclin T1