Cyclin H Antibody (YA482)

Cyclin H Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 38 kDa, targeting to Cyclin H. It can be used for WB, IF-Cell, IF-Tissue, IHC-P, IP, FC assays with tag free, in the background of Human and Mouse.

Cyclin H: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Free

P51946

Predicted band size: 38 kDa; Observed band size: 38 kDa

affinity purified

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

Human, Mouse

WB: 1:1,000-1:5,000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; IP: 1:50; FC: 1:50-1:100

WB, IHC-P, ICC/IF, IP, FC, IF-Tissue

WB, IHC-P, ICC/IF, FC

K562 cell lysate, Jurkat cell lysate, Hela, MCF-7, PC-3M, mouse testis tissue, human colon cancer tissue.

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein within Human Cyclin H aa 230-323 / 323.