CXCR3 Antibody

CXCR3 Antibody is an unconjugated, approximately 40 kDa, rabbit-derived, anti-CXCR3 polyclonal antibody. CXCR3 Antibody can be used for: WB expriments in human, mouse, rat, and predicted: dog, pig, cow, rabbit, guinea pig background without labeling.

The CXCR3 Protein functions as a receptor for the C-X-C chemokines CXCL9, CXCL10, and CXCL11, mediating the proliferation, survival, and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Additionally, it binds to CCL21, potentially promoting cell chemotaxis response. CXCR3 also serves as a receptor for the C-X-C chemokine CXCL4, playing a role in mediating the inhibitory activities of CXCL9, CXCL10, and CXCL11 on the proliferation, survival, and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway. Interestingly, CXCR3 does not promote cell chemotaxis response but, upon interaction with CXCL4 or CXCL10, activates the p38MAPK pathway, contributing to the inhibition of angiogenesis. Notably, overexpression of CXCR3 in renal cancer cells down-regulates the expression of the anti-apoptotic protein HMOX1 and promotes apoptosis, indicating its involvement in modulating cellular processes relevant to cancer biology.

O88410

Predicted band size: 40 kDa; Observed band size: 40 kDa

affinity purified

Cell membrane; Multi-pass membrane protein.

Non-conjugated

Unmodified

AB_3103263

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Dog, Pig, Cow, Rabbit, GuineaPig

WB: 1:500-2000

• 
  Western blot analysis of extracts from 293T (lane 1) and A549 (lane 2) and M-Lymph (lane 3) using CXCR3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse CXCR3: 301-367/367