CSNK2A1 Antibody (YA2833)

CSNK2A1 Antibody (YA2833) is a non-conjugated IgG antibody, targeting CSNK2A1, with a predicted molecular weight of 45 kDa (observed band size: 45 kDa). CSNK2A1 Antibody (YA2833) can be used for WB experiment in human, mouse, rat background.

The catalytic subunit of CSNK2A2 protein functions as a core component of a constitutively active serine/threonine-protein kinase complex, showcasing a broad substrate specificity for acidic residues C-terminal to the phosphorylated serine or threonine. Operating as a regulatory node, CSNK2A2 plays a pivotal role in diverse cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. It contributes to the p53/TP53-dependent spindle assembly checkpoint during mitosis, maintaining cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Additionally, CSNK2A2 is implicated in apoptotic regulation, phosphorylating key players like caspases and NOL3. Its influence extends to transcriptional regulation, with direct phosphorylation of RNA polymerases and numerous transcription factors. In the intricate landscape of cellular signaling, CSNK2A2 participates in Wnt signaling, acts as an ectokinase for extracellular proteins, and phosphorylates various proteins involved in the viral life cycles of diverse viruses. Moreover, it plays a crucial role in the circadian clock function and contributes to the formation of a membraneless compartment by phosphorylating FMR1. Overall, CSNK2A2 emerges as a central orchestrator of cellular responses to diverse stimuli, showcasing its significance in maintaining cellular homeostasis.

P68400

Predicted band size: 45 kDa; Observed band size: 45 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105204

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using CSNK2A1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human CSNK2A1